CST, 65563S, Granzyme B (D2H2F) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying Granzyme B. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Granzyme B (D2H2F) Rabbit Monoclonal Antibody (PE Conjugate) (CST #65563) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Granzyme B (D2H2F) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total Granzyme B protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Granzyme B protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Alternate Names C11; Cathepsin G-like 1; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA-1; CTLA1; CTSGL1; cytotoxic serine protease B; Cytotoxic T-lymphocyte proteinase 2; fragmentin 2; Fragmentin-2; GRAB; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GRB; GZMB; HLP; Human lymphocyte protein; Lymphocyte protease; SECT; T-cell serine protease 1-3E Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG