CST, 65575S, PDP1 (D8Y6L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PDP1 (D8Y6L) Rabbit Monoclonal Antibody (CST #65575) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PDP1 (D8Y6L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PDP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu327 of human PDP1 protein. Background Pyruvate dehydrogenase (PDH) catalyzes decarboxylation of the glycolytic intermediate pyruvate to acetyl-CoA (1). Acetyl-CoA is further metabolized in the tricarboxylic acid (TCA) cycle to generate ATP and NADH (1). Pyruvate dehydrogenase kinase 1 (PDHK1) phosphorylates PDH to suppress its activity, while pyruvate dehydrogenase phosphatase 1 (PDP1) dephosphorylates PDH to enhance its activity (1). Phosphorylation of PDP1 at Tyr94 inhibits PDP1 and has been shown to be present in a variety of cancer cell lines and primary human leukemia cells (2). Alternate Names [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; FLJ32517; FLJ56179; MGC119646; PDH; PDP; PDP 1; PDP1; PDPC; PDPC 1; PPM2A; PPM2C; Protein phosphatase 2C; protein phosphatase 2C, magnesium-dependent, catalytic subunit; protein phosphatase, Mg2+/Mn2+ dependent 2A; pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase; Pyruvate dehydrogenase phosphatase catalytic subunit 1; pyruvate dehyrogenase phosphatase catalytic subunit 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG