CST, 65616T, HMCES (F4E4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HMCES. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, HMCES (F4E4Q) Rabbit Monoclonal Antibody (CST #65616) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HMCES (F4E4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total HMCES protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys349 of human HMCES protein. Background HMCES is an abasic site processing protein belonging to the SOS-response associated peptidase (SRAP) family (1,2). In DNA, loss of a base or nucleotide generates an abasic or apurinic/apyrimidinic (AP) site, one of the most common DNA lesions (1,2). HMCES plays a critical role in recognizing these abasic (AP) sites (1,2). HMCES directly binds proliferating cell nuclear antigen (PCNA) and single-stranded DNA (ssDNA) at replication forks, forming covalent cross-links to promote error-free genome repair (1-3). The HMCES DNA-protein cross-link (DPC) prevents translesion DNA synthesis and endonuclease activity, thereby stopping the generation of mutations and double-stranded DNA (dsDNA) breaks (2). Following this action, the HMCES-DPC is degraded by the proteasome or self-reversed (2,3). In dsDNA, AP sites are repaired via the base excision repair (BER) pathway (1-3). During somatic hypermutation (SHM) in B cells, HMCES suppresses deletions within immunoglobulin (Ig) genes but allows other types of point mutations to occur, resulting in antigen-specific high-affinity antibodies (4). HMCES deficiency impairs class switch recombination (CSR) in B cells, leading to weakened antibody production (5). In APOBEC3A-expressing tumors, disruption of HMCES may increase the tumor's sensitivity to therapies such as ionizing radiation (IR), oxidative stress, and ATR inhibition (6). Alternate Names 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine binding, ES cell specific; Abasic site processing protein HMCES; C3orf37; DC12; Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; HMCES; MGC111075; Peptidase HMCES; putative endonuclease HMCES; putative peptidase SRAPD1; SOS response associated peptidase domain containing 1; SRAP domain-containing protein 1; SRAPD1; UPF0361 protein C3orf37 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG