CST, 65631SF, DDX4 (D10C5) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying DDX4. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, DDX4 (D10C5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65631) is ready to ship. Product Usage Information This product is the carrier free version of product #8761. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8761 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity DDX4 (D10C5) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total DDX4 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DDX4 protein. Background DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1). Alternate Names DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; DEAD-box helicase 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG