CST, 65653S, Rad51 (F1G6C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rad51. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Rad51 (F1G6C) Rabbit Monoclonal Antibody (CST #65653) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Rad51 (F1G6C) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rad51 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Rad51 protein. Background DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways: nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human diseases, including cancer (reviewed in 1). DSBs that arise during S or G2 phase are repaired via homologous recombination, using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homolog of RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA) (4). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D), and they all appear to be required for efficient HR (5). Researchers have found that mutations in the gene may be related to breast cancer risk (6). Some studies have implicated Rad51 as a potential marker for pancreatic cancer (7). Alternate Names BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51 homolog 1; FANCR; hRAD51; HsRAD51; HsT16930; MRMV2; RAD51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog A; RAD51 homolog protein; RAD51 recombinase; RAD51A; RECA; RecA-like protein; RecA, E. coli, homolog of; recombination protein A Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG