CST, 65707S, VWF (D8L8G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VWF. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, VWF (D8L8G) Rabbit Monoclonal Antibody (CST #65707) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 Storage For a carrier free (BSA and azide free) version of this product see product # 39413 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity VWF (D8L8G) Rabbit Monoclonal Antibody recognizes endogenous levels of total VWF protein. This antibody recognizes both the VWF precursor and mature VWF. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human VWF protein. Background VWF (Von Willebrand factor) is a multimeric plasma glycoprotein that promotes adhesion of platelets to sites of vascular injury (1). Mature circulating VWF is made up of disulfide-bonded multimers that are in a complex with factor VIII (2). VWF is stored in secretory Weibel-Palade bodies in endothelial cells (3,4). It is synthesized as a large precursor protein and undergoes extensive posttranslational modifications including dimerization in the endoplasmic reticulum followed by cleavage of the pro-peptide and multimerization in the Golgi apparatus (3,4). VWF is important in hemostasis, and genetic defects in the structure and modification of VWF can cause von Willebrand disease (VWD), the most common congenital bleeding disorder in humans (5). Alternatively, increased levels of VWF have been shown to be involved in acute coronary thrombosis and are a clinical risk marker for atherosclerosis (6). VWF has also been shown to have a role in inflammation, functioning as an adhesive site for a variety of leukocyte subsets (7). Through siRNA experiments and the use VWF-deficient mice, it has also been shown that VWF regulates angiogenesis (8). Alternate Names coagulation factor VIII VWF; F8VWF; von Willebrand antigen 2; von Willebrand antigen II; von Willebrand factor; VWD; VWF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 310, 460 Source/Isotype: Rabbit IgG