CST, 6571T, ACO2 (D6D9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ACO2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, ACO2 (D6D9) Rabbit Monoclonal Antibody (CST #6571) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ACO2 (D6D9) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACO2 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly540 of human ACO2 protein. Background Aconitase 2 (ACO2) catalyzes the conversion of citrate to isocitrate via cis-aconitate in the second step of the tricarboxylic acid (TCA) cycle (1,2). ACO2 is also an important regulator of iron homeostasis within cells (1-4). In addition, research studies have shown that this enzyme is deficient in the mitochondrial disease Friedreich's Ataxia (4,5). Alternate Names ACO2; ACON; Aconitase; aconitase 2; aconitase 2, mitochondrial; Aconitate hydratase, mitochondrial; ACONM; Citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; HEL-S-284; ICRD; MGC20605; MGC33908; mitochondrial aconitase; OCA8; OPA9 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG