CST, 65763S, ETV4 (E1W1G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ETV4. Validated for WB,WB,IP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, ETV4 (E1W1G) Rabbit Monoclonal Antibody (CST #65763) is ready to ship. Product Usage Information For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Chromatin IP: 1:200 CUT&RUN: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN Specificity / Sensitivity ETV4 (E1W1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETV4 protein. This antibody does not cross-react with ETV5 protein, and based on observed expression is not predicted to cross-react with ETV1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human ETV4 protein. Background ETS translocation variant 4 (ETV4, PEA3, E1AF) is a member of the PEA3 (polyomavirus enhancer activator 3) subfamily of the E26 transformation-specific (ETS) DNA-binding transcription factors, which commonly bind a core DNA sequence of 5'-GGA(A/T)-3' (1,2). The PEA3 subfamily of ETV1, ETV4, and ETV5 are considered oncogenic transcription factors due to their overexpression and promotion of tumorigenesis in numerous cancers (2,3). ETV4 can be activated via the RAS-RAF-MEK-ERK pathway (2,4) or the PI3K/Akt pathway (5), and loss-of-function mutations of PEA3-family repressors such as CIC can increase ETV4 expression independent of either pathway (2,6). Though rare, 5' chromosomal fusions to ETV4 are drivers of metastasis in prostate cancer (7) and Ewing sarcoma (8). ETV4 regulates the expression of genes that control several developmental processes, including cell growth, differentiation, proliferation, survival, and cell cycle progression (9-11). Alternate Names Adenovirus E1A enhancer-binding protein; E1A enhancer binding protein; E1A-F; E1AF; ETS translocation variant 4; ets variant 4; ets variant gene 4 (E1A enhancer binding protein, E1AF); ets variant gene 4 (E1A enhancer-binding protein, E1AF); ETS variant transcription factor 4; ETV4; EWS protein/E1A enhancer binding protein chimera; PEA3; PEAS3; Polyomavirus enhancer activator 3 homolog; polyomavirus enhancer activator-3; Protein PEA3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 54-61 Source/Isotype: Rabbit IgG