CST, 65766SF, LbCpf1/Cas12a (Strain ND2006) (2D5-6G11) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LbCpf1 (Strain ND2006). Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),ELISA+. Highly specific and rigorously validated in-house, LbCpf1/Cas12a (Strain ND2006) (2D5-6G11) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #65766) is ready to ship. Product Usage Information This product is the carrier free version of product #91982. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 91982 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LbCpf1/Cas12a (Strain Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of LbCpf1/Cas12a (Strain ) protein. Background CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from and ) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4). LbCpf1 (Strain )/Cas12a is a Cpf1/Cas12a enzyme derived from (5,6). Alternate Names Cpf1; LbCpf1 Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 143 Source/Isotype: Mouse IgG1