CST, 65778SF, SOD1 (E4G1H) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SOD1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, SOD1 (E4G1H) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65778) is ready to ship. Product Usage Information This product is the carrier-free version of product #37385. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product #www.cellsignal.com/products/primary-antibodies/sod1-e4g1h-xp-rabbit-mab/37385?_=1657551366122&Ntt=37385&tahead=true">37385. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SOD1 (E4G1H) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SOD1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOD1 protein. Background SOD1, Cu/Zn superoxide dismutase, is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen (1). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space. The SOD1 gene locus is on chromosome 21 in a region affected in Down Syndrome (2). In addition, over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons (3-5). Despite the fact that SOD1 helps to eliminate toxic reactive species, its mutations in ALS have been described as gain-of-function (5). The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear. Mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight species that aggregate and end up in proteinaceous inclusions (5). Alternate Names ALS; ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; homodimer; hSod1; indophenoloxidase A; IPOA; SOD; SOD, soluble; SOD1; SODC; STAHP; superoxide dismutase; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; superoxide dismutase, cystolic Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 16-18 Source/Isotype: Rabbit IgG