CST, 65874S, Tox2 (E3N3G) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying Tox2. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Tox2 (E3N3G) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #65874) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Tox2 (E3N3G) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu343 of human Tox2 protein. Background Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox2 is expressed by natural killer (NK) cells, T follicular helper (Tfh) cells, and exhausted CD8 T cells (3,4). Tox2 and BCL6 play a critical role in Tfh cell development by generating a transcriptional feed-forward loop that drives the Tfh program (5). Tox2 induction plays a role in driving CD8 T cell exhaustion and is being investigated as a therapeutic target for cancer immunotherapy (6). Alternate Names C20orf100; dJ1108D11.2; dJ495O3.1; GCX-1; GCX1; granulosa cell HMG box 1; Granulosa cell HMG box protein 1; granulosa cell HMG-box protein 1; MGC15880; TOX high mobility group box family member 2; TOX2 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG