CST, 65880T, ATF-6 (D4Z8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATF6a. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ATF-6 (D4Z8V) Rabbit Monoclonal Antibody (CST #65880) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ATF-6 (D4Z8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-6 alpha protein. This antibody does not detect ATF-6 beta protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-6 alpha protein. Background Activating Transcription Factor 6 (ATF-6) is a transmembrane glycoprotein of the endoplasmic reticulum (ER). Upon accumulation of improperly folded proteins in the ER, ATF-6 is cleaved liberating a 50 kDa amino-terminal fragment that translocates to the nucleus (1, 2). There, ATF-6 activates transcription of ER chaperones and components of ER-associated degradation such as GRP78, XBP-1, and CHOP (3-5). Alternate Names ACHM7; activating transcription factor 6; Activating transcription factor 6 alpha; ATF6; ATF6-alpha; ATF6A; cAMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 90-100 Source/Isotype: Rabbit IgG