CST, 65893S, TRPV4 Antibody

Polyclonal Antibody for studying TRPV4. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TRPV4 Antibody (CST #65893) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRPV4 Antibody recognizes endogenous levels of total TRPV4 protein. This antibody is predicted to detect all isoforms of TRPV4 reported in Uniprot, with the exception of TRPV4-SV (Isoform 3). Species Reactivity: Human, Mouse, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TRPV4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background TRPV4 is a member of the transient receptor potential vanilloid (TRPV) family of ion channels, and functions as a Ca -permeant non-selective cation channel. TRPV4 channels are expressed in many cell types, with particular abundance in sensory and spinal neurons (1). TRPV4 channels play a role in maintaining cellular homeostasis, by facilitating transmembrane Ca transport in response to various stimuli, including thermal stress, fatty acid metabolites, and hypotonicity (2). Mutations in the gene have consequently been attributed to a variety of pathological conditions. For example, constitutively active TRPV4 mutants can lead to excess Ca influx, resulting in toxicity and degeneration of peripheral nerves (3). TRPV4-dependent Ca influx was also shown to mediate strain-induced and TGFβ1-induced epithelial-mesenchymal transition (EMT), suggesting a mechanistic role for TRPV4-mediated Ca transport in fibrosis and oncogenesis (4). Consistent with this, studies in capillary endothelial cells showed that mechanical strain-induced Ca influx through TRPV4 promote focal adhesion and stress fiber remodeling, mediated specifically through integrins, PI3K, and downstream kinases including Rho and ROCK (5). Alternate Names BCYM3; CMT2C; HMSN2C; Osm-9-like TRP channel 4; OSM9-like transient receptor potential channel 4; osmosensitive transient receptor potential channel 4; OTRPC4; SMAL; SPSMA; SSQTL1; Transient receptor potential cation channel subfamily V member 4; transient receptor potential cation channel, subfamily V, member 4; Transient receptor potential protein 12; TRP12; TRPV4; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; vanilloid receptor-related osmotically activated channel; Vanilloid receptor-related osmotically-activated channel; VR-OAC; VRL-2; VRL2; VROAC Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 95-102 SOURCE: Rabbit