CST, 65896SF, MIST1/bHLHa15 (D7N4B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying bHLHa15/MIST1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, MIST1/bHLHa15 (D7N4B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65896) is ready to ship. Product Usage Information This product is the carrier free version of product #14896. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14896 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MIST1/bHLHa15 (D7N4B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MIST1/bHLHa15 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln181 of human MIST1/bHLHa15 protein. Background Class A basic helix-loop-helix protein 15 (MIST1, bHLHa15) is a highly conserved basic helix loop helix family transcription factor that binds E-box motifs and regulates the expression of developmentally regulated genes (1). MIST1 can bind DNA as a homodimer, or may heterodimerize with other bHLH proteins to regulate target gene expression (1). MIST1 is expressed in an array of tissues, including salivary glands, stomach, small intestine, and the pancreas, but is generally restricted to secretory cell subtypes (2). In the pancreas, MIST1 is essential for the maturation, maintenance, and function of acinar cells (3). In gastric chief cells, MIST1 regulates the expression of RAB26 and RAB3D, two GTPases that function to regulate secretory granule formation (4). Loss of MIST1 in gastric chief cells may be a potential marker of gastric epithelial neoplasia (5). Alternate Names basic helix-loop-helix domain containing, class B, 8; basic helix-loop-helix family member a15; basic helix-loop-helix family, member a15; BHA15; BHLHA15; BHLHB8; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; class II bHLH protein MIST1; MIST-1; MIST1; Muscle, intestine and stomach expression 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG