CST, 65911SF, MAP2 (D5G1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MAP2. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, MAP2 (D5G1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #65911) is ready to ship. Product Usage Information This product is the carrier-free version of product #8707. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8707 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MAP2 (D5G1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MAP2 protein. Non-specific labeling of the pancreas and macrophages in mouse liver tissue may be observed by immunofluorescence. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAP2 protein. Background Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3). Alternate Names DKFZp686I2148; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; microtubule associated protein 2; Microtubule-associated protein 2; MTAP2 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 75, 82, 280 Source/Isotype: Rabbit IgG