CST, 65915S, Olig2 (E6G6Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OLIG2. Validated for WB,WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Olig2 (E6G6Q) Rabbit Monoclonal Antibody (CST #65915) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 71735 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Olig2 (E6G6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total Olig2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Olig2 protein. Background Olig2 is a basic helix-loop-helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the central nervous system (1,2). Olig2 has been shown to play a role in Purkinje cell Specification in the early cerebellum (3). Olig2 has also been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (4,5). Alternate Names basic domain, helix-loop-helix protein, class B, 1; BHLHB1; BHLHE19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; OLIG2; Oligo2; oligodendrocyte lineage transcription factor 2; Oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C binding protein 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG