CST, 65923T, CD64/FcgammaRIA (E6I4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcGR1A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD64/FcgammaRIA (E6I4Z) Rabbit Monoclonal Antibody (CST #65923) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD64/FcgammaRIA (E6I4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD64/FcγRIA protein. This antibody does not cross-react with FcγRIB protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human CD64/FcγRIA protein. Background CD64/FcγRI (high affinity IgG Fc gamma RI) is an activating Fcγ receptor in the immunoglobulin superfamily, predominantly found on macrophages and monocytes (1-4). CD64 has a high affinity for the antibody-constant region of IgG, with three extracellular Ig-like domains (3). After binding IgG, CD64 interacts with an accessory chain known as the FcR common γ chain (FcRγ), which possesses an immunoreceptor tyrosine-based activation motif (ITAM) that is necessary for triggering cellular activation (2,3). Alternate Names CD64; CD64A; Fc fragment of IgG receptor Ia; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FCG1; FcgammaRIa; FCGR1; FCGR1A; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-50, 60-80 Source/Isotype: Rabbit IgG