CST, 66000T, SENP6 (F2K4K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SENP6. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SENP6 (F2K4K) Rabbit Monoclonal Antibody (CST #66000) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SENP6 (F2K4K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SENP6 protein. This antibody may detect a band of unknown origin at 80 kDa in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly842 of human SENP6 protein. Background SUMOylation is a post-translational modification (PTM) that involves the covalent attachment of small ubiquitin-related modifier (SUMO) proteins to substrate proteins. SUMOylation is a reversible process that can be erased by SUMO-specific proteases (SENPs) (1). SENP6 is a critical factor for cell cycle progression and survival (2-4). SENP6 has been shown to deconjugate SUMO2/3 polymers on a group of DNA damage response proteins and is required for maintaining genomic stability (5). Alternate Names 2810017C20Rik; FLJ11355; FLJ11887; KIAA0389; KIAA0797; SENP6; Sentrin-specific protease 6; Sentrin/SUMO-specific protease SENP6; SSP1; SUMO specific peptidase 6; SUMO-1-specific protease 1; SUMO1/sentrin specific peptidase 6; SUMO1/sentrin specific protease 6; SUSP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG