CST, 66006SF, ELAVL1/HuR (D9W7E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ELAVL1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, ELAVL1/HuR (D9W7E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #66006) is ready to ship. Product Usage Information This product is the carrier free version of product #12582. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12582 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ELAVL1/HuR (D9W7E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ELAVL1/HuR protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1/HuR protein. Background The ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC and ELAVL4/HuD (1). ELAVL1/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3' UTRs to stabilize mRNAs (3, 4). ELAVL1/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1/HuR interacts with microRNAs (miRNAs) (7). Alternate Names ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like RNA binding protein 1; ELAV-like protein 1; ELAV1; ELAVL1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; Hu-antigen R; Hua; HUR; MelG Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG