CST, 66102S, CD96 (E1Y2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD96 mouse. Validated for WB,IF,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD96 (E1Y2Z) Rabbit Monoclonal Antibody (CST #66102) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD96 (E1Y2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD96 protein. This antibody does not cross-react with human CD96 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse CD96 protein. Background Cluster of Differentiation 96 (CD96), also known as T cell-activated increased late expression protein (TACTILE), is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily (1-3). CD96 is expressed on peripheral T cells and natural killer (NK) cells, and is strongly upregulated on these cell types after activation (1,4). It is also expressed on transformed T cells, and at very low levels on activated B cells (1,5,6). CD96 interacts with the receptor ligand PVR (CD155), and is involved in adhesion of activated T and NK cells to target cells during the late phase of the immune response (2,4). CD96 competes with DNAM-1 (CD226) for binding to PVR (CD155) in order to negatively regulate T and NK cell activity, and thus blockade of CD96 in order to induce anti-tumor immune responses is investigated for potential immunotherapeutic intervention in cancer (7-9). Alternative splicing occurs at this locus, and two transcript variants encoding distinct isoforms with variable functions have been identified (1,10). Alternate Names 1700109I12Rik; Cd96; CD96 antigen; Cell surface antigen CD96; OTTMUSP00000018537; T cell activation, increased late expression; T cell-activated increased late expression protein; T-cell surface protein tactile; TACT; Tactile Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 100-200 Source/Isotype: Rabbit IgG