CST, 66115SF, NTAL/LAB (D7I2B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NTAL. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, NTAL/LAB (D7I2B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #66115) is ready to ship. Product Usage Information This product is the carrier free version of product #11986. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 11986 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NTAL/LAB (D7I2B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NTAL/LAB protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly222 of human NTAL/LAB protein. Background Non-T cell activation linker (NTAL)/linker for activation of B cells (LAB) is a small transmembrane adaptor protein associated with glycolipid-enriched membrane fractions (1,2). NTAL/LAB is also known as LAT2 (linker for activation of T cells 2), WBSCR5, WBS15, and WBSCR15 (Williams-Beuren syndrome chromosome region 15 protein). It is expressed in B cells, monocytes, mast cells, and natural killer cells, but not in resting T cells (3). Upon activation of several receptors, NTAL/LAB becomes tyrosine-phosphorylated and recruits signaling molecules such as GRB2 and c-Cbl into receptor signaling complexes (4-6). Alternate Names HSPC046; LAB; LAT2; linker for activation of B cells; Linker for activation of B-cells; linker for activation of T cells family member 2; linker for activation of T cells family, member 2; linker for activation of T cells, transmembrane adaptor 2; Linker for activation of T-cells family member 2; Membrane-associated adapter molecule; non-T cell activation linker; Non-T-cell activation linker; NTAL; WBS15; WBSCR15; WBSCR5; Williams-Beuren syndrome chromosomal region 15 protein; Williams-Beuren syndrome chromosomal region 5 protein; Williams-Beuren syndrome chromosome region 15; Williams-Beuren syndrome chromosome region 5; WSCR5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG