CST, 66310T, USP15 (D1K6S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USP15. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, USP15 (D1K6S) Rabbit Monoclonal Antibody (CST #66310) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity USP15 (D1K6S) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP15 protein. This antibody does not cross-react with either USP4 or USP11 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human USP15 protein. Background Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs), respectively (1,2). DUBs are categorized into five subfamilies based on catalytic domain structure: USP, UCH, OTU, MJD, and JAMM. Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a USP subfamily deubiquitinating enzyme with similar domain structure to the paralogous DUBs, USP4, and USP11. The gene is amplified in glioblastoma and other solid tumors and its high expression correlates with a poor prognosis (3,4). Research studies demonstrate that USP15 is a positive regulator of oncogenic TGF-β signaling and that USP15 deubiquitinates monoubiquitinated R-SMADs to enhance target gene promoter binding (5). USP15 also promotes oncogenic TGF-β signaling by opposing SMURF2-mediated ubiquitination of the type I TGF-β receptor, which facilitates receptor stabilization (3,4). USP15 contributes to oncogenesis by negatively regulating T cell-mediated antitumor responses through the deubiquitination and stabilization of the E3 ubiquitin ligase MDM2. This observation supports USP15 as a potential target for cancer therapeutics (6). Alternate Names Deubiquitinating enzyme 15; KIAA0529; MGC131982; MGC149838; MGC74854; Ubiquitin carboxyl-terminal hydrolase 15; ubiquitin specific peptidase 15; ubiquitin specific protease 15; Ubiquitin thioesterase 15; ubiquitin thiolesterase 15; Ubiquitin-specific-processing protease 15; UBP15; Unph-2; Unph4; USP15 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG