CST, 66362S, Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (CUT&RUN)

Monoclonal Antibody for studying . Validated for CUT&RUN. Highly specific and rigorously validated in-house, Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (CUT&RUN) (CST #66362) is ready to ship. Product Usage Information Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background may result if excessive amounts of rabbit IgG isotype control is used. This antibody has been tested and validated using CUT&RUN Assay Kit # 86652 . Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Protocol Available protocols: CUT&RUN Specificity / Sensitivity Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (CUT&RUN) is not directed against any known antigen. This antibody functions as an isotype control for rabbit IgG monoclonal antibodies in the CUT&RUN assay. Background Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to F receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. Specification Concentration: 100 µg/ml SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG