CST, 66382S, Tapasin (E6P2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TAPBP. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Tapasin (E6P2Z) Rabbit Monoclonal Antibody (CST #66382) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37427 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Tapasin (E6P2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tapasin. This antibody does not cross-react with Tapasin-R protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human Tapasin protein. Background TAP-associated glycoprotein (Tapasin/TAPBP) is a type-I transmembrane ER glycoprotein encoded by an MHC-linked gene. Tapasin plays a critical role in the MHC class I-restricted antigen processing and presentation pathway. Research studies have demonstrated that Tapasin is an IFNγ-inducible component of the TAP-1/2 peptide transporter complex and, within the ER lumen, facilitates the optimization of the peptide repertoire that is loaded onto MHC class I molecules (1-5). Downregulation of Tapasin and MHC class I expression has been observed in human tumors and serves as a prominent mechanism of tumor immune escape (6-9). Alternate Names NGS-17; NGS17; TAP binding protein; TAP binding protein (tapasin); TAP-associated protein; TAP-binding protein; TAPA; Tapasin; TAPBP; TPN; TPSN Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG