CST, 66423S, Dectin-1 (E1X3Z) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying Dectin-1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Dectin-1 (E1X3Z) Rabbit Monoclonal Antibody (PE Conjugate) (CST #66423) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Dectin-1 (E1X3Z) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total Dectin-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Dectin-1 protein. Background Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9). Alternate Names Beta-glucan receptor; BGR; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain containing 7A; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; CANDF4; CD369; CLC7A; CLEC7A; CLECSF12; DC-associated C-type lectin 1; Dectin-1; DECTIN1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; SCARE2 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG