CST, 66433T, TBC1D1 (D2Y8M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TBC1D1 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TBC1D1 (D2Y8M) Rabbit Monoclonal Antibody (CST #66433) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TBC1D1 (D2Y8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total TBC1D1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val796 of mouse TBC1D1 protein. Background TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified in skeletal muscle (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5). Alternate Names 1110062G02Rik; AI385682; AW555803; Kiaa1108; MGC106104; MGC144097; mKIAA1108; Nob; Nob1; Nobq1; Tbc1; TBC1 domain family member 1; TBC1 domain family, member 1; Tbc1d1; TBCD1; tre-2/USP6, BUB2, cdc16) domain family, member 1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG