CST, 66470S, Pyruvate Carboxylase Antibody

Polyclonal Antibody for studying Pyruvate Carboxylase. Validated for Western Blotting. Highly specific and rigorously validated in-house, Pyruvate Carboxylase Antibody (CST #66470) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Pyruvate Carboxylase Antibody recognizes endogenous levels of total pyruvate carboxylase protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human pyruvate carboxylase protein. Antibodies are purified by peptide affinity chromatography. Background Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate in an anaplerotic pathway to replenish TCA cycle intermediates. It is also critical in regulating gluconeogenesis in the liver (1). Studies show that the increase of PC flux contributes to the induction of hepatic gluconeogenesis by glucagon (2). In addition, high-fat diet is shown to promote hepatic glucose uptake and induce PC activity in the liver (3). Loss of hepatic PC prevents diet-induced hyperglycemia and insulin resistance. Furthermore, this enzyme is also required for antioxidant defenses (1). Alternate Names PC; PCB; PYC; pyruvate carboxylase; Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 130 SOURCE: Rabbit