CST, 66546S, cGAS (D1D3G) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying cGAS. Validated for Western Blotting. Highly specific and rigorously validated in-house, cGAS (D1D3G) Rabbit Monoclonal Antibody (Biotinylated) (CST #66546) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity cGAS (D1D3G) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total cGAS protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala19 of human cGAS protein. Background Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5). Alternate Names 2'3'-cGAMP synthase; C6orf150; CF150; cGAMP synthase; cGAS; Cyclic GMP-AMP synthase; FLJ39829; h-cGAS; LOC115004; Mab-21 domain containing 1; Mab-21 domain-containing protein 1; MB21D1; MGC131892; MGC142166; MGC142168; protein MB21D1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG