CST, 66548S, NAT10 Antibody

Polyclonal Antibody for studying NAT10. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NAT10 Antibody (CST #66548) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NAT10 Antibody recognizes endogenous levels of total NAT10 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NAT10 protein. Antibodies are purified by peptide affinity chromatography. Background N-acetyltransferase 10 (NAT10), also known as human N-acetyltransferase-like protein (hALP), is an acetyltransferase that increases telomerase activity through the activation of the TERT promoter (1). NAT10 localizes to the midbody in late phase mitosis to help achieve cytokinesis (2). It has been found that DNA damage can actually increase NAT10-dependent acetylation of alpha-tubulin, resulting in increased stability of the protein (2,3). This increased stability of microtubules has been linked to the sequestration of the nuclear import factor transportin-1 in the premature aging disease, progeria (4,5). NAT10 can also function as an acetyltransferase for both rRNA and mRNA (6-8). The acetylation of the 18 S rRNA helps in rRNA processing and ribosome biogenesis, and this process can be disrupted by opposing activity of the deacetylase SirT1 in response to stress (6,9). mRNA acetylation by NAT10 plays a role in translation efficiency and mRNA stability (7,8). NAT10 is overexpressed in many different cancer types, making it a good potential therapeutic target (10-12). Alternate Names 18S rRNA cytosine acetyltransferase; ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; hALP; KIAA1709; Kre33; N-acetyltransferase 10; N-acetyltransferase 10 (GCN5-related); N-acetyltransferase-like protein; NAT10; NET43; RNA cytidine acetyltransferase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit