CST, 66564S, PARG (D4E6X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PARG. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PARG (D4E6X) Rabbit Monoclonal Antibody (CST #66564) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PARG (D4E6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total PARG protein. This antibody specifically recognizes PARG isoform 1 (UniProt #Q86W56-1) and does not recognize other PARG isoforms. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PARG protein. Background Poly (ADP-ribose) glycohydrolase (PARG) is an enzyme that hydrolyzes poly (ADP-ribose) (PAR) formed by members of the PAR polymerase (PARP) enzyme family. Poly (ADP)-ribosylation is a post-translational modification that is catalyzed by PARP proteins. This modification involves polymerization of ADP-ribose from NAD to target proteins, such as histones and transcription factors, and plays a wide range of biological roles, including the response to DNA damage and transcriptional regulation (1,2). The mammalian PARG enzyme that catalyzes the removal of this modification exists as multiple isoforms. PARG isoforms 1-3 shuttle between the nucleus and cytoplasm and are responsible for most of the PARG activity. The smaller isoforms 4 and 5 reside in the cytoplasm (3-5). Research studies link altered PAR metabolism to inflammatory and autoimmune diseases, as well as neuronal degeneration (6-8). PARG inhibitors that increase PAR levels may sensitize cells to cancer treatments (e.g., cisplatin) and may help in the development of cancer therapies (9). Alternate Names FLJ60456; mitochondrial poly(ADP-ribose) glycohydrolase; PARG; PARG99; poly (ADP-ribose) glycohydrolase; Poly(ADP-ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG