CST, 66798SF, PODXL (E8O1S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PODXL. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PODXL (E8O1S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #66798) is ready to ship. Product Usage Information This product is the carrier free version of product #55601. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 55601 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PODXL (E8O1S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PODXL protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PODXL protein. Background Podocalyxin is an O-glycosylated and sialylated membrane protein that is closely similar to CD34, the well characterized marker for hematopoietic stem cells (1). In association with several intracellular binding partners, podocalyxin contributes to adhesion and cell morphology (2-4). Podocalyxin is expressed in hematopoietic tissues during stages of active hematopoietic expansion in embryonic development and in more restricted, mainly megakaryocytic lineages in the adult (1,4). Expression also occurs in kidney podocytes, both in embryo and adult, where podocalyxin is essential for organ development and proper kidney function (5-7). A few other cell types that express podocalyxin include some specialized vascular endothelia (8,9) and a subset of neurons (10). It is also aberrantly expressed in a diverse number of cancers, including breast, testicular, pancreatic cancers, and in leukemia (11). Expression is often correlated with poor outcome (11). Alternate Names GCTM-2 antigen; gp135; Gp200; MGC138240; PC; PCLP; PCLP-1; PCLP1; PDX; Podocalyxin; podocalyxin like; podocalyxin-like; Podocalyxin-like protein 1; PODXL; PODXL1; TRA-1-60 antigen; TRA-1-61 antigen; TRA-1-80 antigen; TRA-1-81 antigen Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG