CST, 66849S, NCOA4 (E8H8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NCOA4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NCOA4 (E8H8Z) Rabbit Monoclonal Antibody (CST #66849) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NCOA4 (E8H8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total NCOA4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly213 of human NCOA4 protein. Background Nuclear Receptor Coactivator 4 (NCOA4), also known as ARA70 or ELE1, was initially discovered as a transcriptional coactivator of androgen receptor (1,2). NCOA4 has also been implicated in regulation of peroxisome proliferator-activated receptor gamma and alpha, as well as estrogen receptor alpha (3-5). NCOA4 plays an autophagic role as a cargo receptor by shuttling ferritin to the lysosome in order to maintain iron homeostasis, which ultimately controls heme synthesis and red blood cell development (6-9). The gene has been shown to undergo a fusion event with the RET proto-oncogene, forming RET/PTC3 in thyroid and other types of cancers (10-13). Alternate Names 70 kDa androgen receptor coactivator; 70 kDa AR-activator; Androgen receptor coactivator 70 kDa protein; androgen receptor coactivator, 70-kD; Androgen receptor-associated protein of 70 kDa; ARA70; DKFZp762E1112; ELE1; NCoA-4; NCOA4; Nuclear receptor coactivator 4; papillary thyroid carcinoma 3; PTC3; ret fused; RET-activating gene ELE1; Ret-activating protein ELE1; RFG Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG