CST, 67041S, Phospho-ACE2 (Tyr781) Antibody

Polyclonal Antibody for studying ACE2 (Tyr781) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-ACE2 (Tyr781) Antibody (CST #67041) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-ACE2 (Tyr781) Antibody recognizes endogenous levels of ACE2 protein only when phosphorylated at Tyr781. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr781 of human ACE2 protein. Antibodies are purified by peptide affinity chromatography. Background ACE2 is a carboxypeptidase that catalyses the conversion of angiotensin I to angiotensin 1-9, or of angiotensin II to the vasodilator angiotensin 1-7 (1). ACE2 is a critical component in the renin-angiotensin system (RAS). ACE2 is predominantly expressed in vascular endothelial cells of the heart and kidney and Leydig and Sertoli cells of the testis (2,3). The unique expression pattern of ACE2 determines its essential role in the regulation of cardiovascular and kidney functions, as well as fertility. ACE2 protein is localized mainly in the extracellular space with its carboxy-terminal end attached to the membrane via its transmembrane domain. Active ACE2 enzyme is secreted by cleavage at the amino terminus. Research studies have shown that ACE2 expression is elevated in human failing heart (4). ACE2 has also been identified as the receptor for SARS and SARS-CoV-2 coronaviruses (5-7). Phosphorylation of ACE2 at Tyr781 has been shown to abolish the interaction of ACE2 with the adaptor protein subunit AP2 μ2, while simultaneously enabling its binding to the SH2 domain of the tyrosine kinase FYN (8,9). These results suggest that remodeling of the actin cytoskeleton may facilitate viral entry after formation of endocytic vesicles, with phosphorylation of Tyr781 acting as a molecular switch. Alternate Names ACE-related carboxypeptidase; ACE2; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; DKFZp434A014; Metalloprotease MPROT15; peptidyl-dipeptidase A; Processed angiotensin-converting enzyme 2; truncated angiotensin converting enzyme 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120-135 SOURCE: Rabbit