CST, 67074S, Mouse EGF Recombinant Protein

Growth Factors and Cytokines for studying EGF mouse in the research area. Storage Mouse EGF Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC. Source / Purification Recombinant mouse EGF was expressed in and is supplied in a lyophilized form. Background The epidermal growth factor (EGF) is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types, including tumor-derived cells (1-3). The EGF and HER/ErbB EGF receptor (EGFR) families have multiple members. EGF binds to HER1/ErbB1 and induces homodimerization or heterodimerization with other HER/ErbB members (1). Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1,2). Alternate Names URG; Urogastrone