CST, 67151S, Integrin alpha-V (F3T8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin Alpha V. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin alpha-V (F3T8T) Rabbit Monoclonal Antibody (CST #67151) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Integrin alpha-V (F3T8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total integrin alpha-V protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human integrin alpha-V protein. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and eight β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Several αV subfamily members, including αVβ3, αVβ5, and αVβ1, are highly expressed in active endothelial cells and cancer cells (3-6), where they play a critical role in angiogenesis and tumor metastasis (7-9). Therefore, interest has focused on αV integrin as a key therapeutic target in cancer treatment (10-12). Alternate Names antigen identified by monoclonal antibody L230; CD51; DKFZp686A08142; Integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; integrin subunit alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITAV; ITGAV; MSK8; Vitronectin receptor; Vitronectin receptor subunit alpha; VNRA; VTNR Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 140, 25 Source/Isotype: Rabbit IgG