CST, 67223S, ICOS (E5F7E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ICOS mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, ICOS (E5F7E) Rabbit Monoclonal Antibody (CST #67223) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 18670 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity ICOS (E5F7E) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse ICOS protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val198 of mouse ICOS protein. Background ICOS (Inducible Co-Stimulator, CD278) is a member of the CD28 family that regulates T cell activity and immune responses (1). The ICOS protein contains an extracellular IgV-like domain, a transmembrane domain, and an intracellular domain with a YMFM motif (1-2). ICOS is primarily expressed on activated CD4 and CD8 T cells (1). Upon binding to its ligand, ICOS potentiates the T cell response to antigen through activation of the PI3K signaling pathway (3). In addition to enhancing T cell activation and proliferation, ICOS plays an important role in the regulation of T follicular helper cells (4). Research studies suggest that ICOS is a potential therapeutic target, and could serve as a prognostic biomarker for neoplastic therapy involving CTLA-4 blockade (5-7). Alternate Names Activation-inducible lymphocyte immunomediatory molecule; Ailim; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CH29-120A16.4; CRP-1; ICOS; inducible T cell co-stimulator; Inducible T-cell costimulator; Ly115; OTTMUSP00000002206; OTTMUSP00000002207 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 22-35 Source/Isotype: Rabbit IgG