CST, 67255S, Neurofilament-M (E7L2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Neurofilament-M. Validated for WB,WB,IP,IHC,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Neurofilament-M (E7L2T) Rabbit Monoclonal Antibody (CST #67255) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53682 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Neurofilament-M (E7L2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Neurofilament-M protein. Non-specific staining was observed in skeletal muscle and some immune cells by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Neurofilament-M protein. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Alternate Names 160 kDa neurofilament protein; NEF3; NEFM; Neurofilament 3; Neurofilament M; neurofilament medium; Neurofilament medium polypeptide; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; NF-M; NFM Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG