CST, 67260T, MEIS1 (E9V1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MEIS1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MEIS1 (E9V1D) Rabbit Monoclonal Antibody (CST #67260) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MEIS1 (E9V1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total MEIS1 protein. This antibody does not cross-react with MEIS2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro51 of human MEIS1 protein. Background Hox, Pbx, and Meis are families of transcription factors that bind DNA via their homeodomains. Members from each family form heterodimers to give rise to complexes with unique DNA binding specificities. Homeodomain-containing proteins are frequently involved in normal developmental processes but can also be associated with tumorigenic states (1). MEIS proteins belong to the three amino acid loop extension (TALE) homeobox-containing transcription factor family. MEIS1 has been associated with leukemogenesis and neuroblastoma (2,3), while MEIS2 is known to play an important role in the transcriptional program that is induced in normal pancreatic development (4) and cardiogenesis (5). Alternate Names Homeobox protein Meis1; leukemogenic homolog protein; Meis homeobox 1; MEIS1; Meis1, myeloid ecotropic viral integration site 1 homolog; myeloid ecotropic viral integration site 1; WUGSC:H_NH0444B04.1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 52, 62 Source/Isotype: Rabbit IgG