CST, 67311S, eIF2 beta (F2R7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF2-beta. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF2 beta (F2R7D) Rabbit Monoclonal Antibody (CST #67311) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity eIF2 beta (F2R7D) Rabbit Monoclonal Antibody recognizes endogenous levels of eIF2β protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly323 of human eIF2β protein. Background eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2β (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2β complex and inhibits the turnover of eIF2β. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). The eIF2β subunit plays a major role in various interactions of the eIF2 trimer with other proteins. eIF2β has been shown to directly interact with other eIF factors, including eIF5, eIF2B, and eIF3 (7-9). eIF2β has also been shown to directly bind mRNA through a C-terminal zinc-finger motif (10,11). Phosphorylation of eIF2β by CK2 promotes the guanine nucleotide exchange activity of eIF2β (12,13). Alternate Names DKFZp686L18198; eIF-2-beta; EIF2; eIF2-beta; EIF2B; EIF2beta; EIF2S2; eukaryotic initiation factor 2-beta; eukaryotic translation initiation factor 2 beta; Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; IF2B; MGC8508; PPP1R67; protein phosphatase 1, regulatory subunit 67 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG