CST, 67330SF, CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD10 mouse. Validated for WB,IHC,IF,IF,F. Highly specific and rigorously validated in-house, CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #67330) is ready to ship. Product Usage Information This product is the carrier free version of product #58145. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 58145 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD10/Neprilysin protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10/Neprilysin protein. Background CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10 GPR77 carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4). Alternate Names 6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; common acute lymphoblastic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; Mme; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG