CST, 67360S, COL17A1 (F5U1Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COL17A1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, COL17A1 (F5U1Z) Rabbit Monoclonal Antibody (CST #67360) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity COL17A1 (F5U1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL17A1 protein. This antibody detects a 62 kDa band, likely a processed N-terminal fragment of COL17A1. Non-specific staining was observed in some peripheral nerves, kidney proximal tubules, and small intestine epithelium by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr102 of human COL17A1 protein. Background Collagen type XVII alpha 1 chain (COL17A1/BP180) is a collagen family hemidesmosome anchorage molecule of basal keratinocytes that promotes stable epidermal-dermal adhesion and wound healing (1). COL17A1 is critical for maintaining keratocyte and hair follicle stem cells, promoting cell motility and increased self-renewing capability (2-4). The protein functions by organizing actin and keratin filament networks via its interaction with intracellular scaffold protein plectin (PLEC) and integrin β4 in the hemidesmosomes (2,5). High expression of COL17A1 is associated with cervical, colorectal, pancreatic, and lung cancers (6-9). It induces a tumor-favorable microenvironment and promotes invasion and metastasis. Conversely, decreased expression of COL17A1 in breast cancer is associated with increased cancer migration and invasion (10). COL17A1 was found to promote cancer dormancy and chemoresistance in colorectal stem cells (11,12). COL17A1 mutations and autoantibodies against COL17A1 can cause blistering skin diseases due to loss of attachment of the epidermis to the underlying basement membrane (13,14). Alternate Names 120 kDa linear IgA dermatosis antigen; 120 kDa linear IgA disease antigen; 180 kDa bullous pemphigoid antigen 2; 97 kDa LAD antigen; 97 kDa linear IgA bullous dermatosis antigen; 97 kDa linear IgA disease antigen; 97-LAD; alpha 1 type XVII collagen; BA16H23.2; bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BP180; BPA-2; BPAG2; Bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); COHA1; COL17A1; Collagen alpha-1(XVII) chain; collagen type XVII alpha 1 chain; collagen XVII, alpha-1 polypeptide; collagen, type XVII, alpha 1; ERED; FLJ60881; KIAA0204; LABD97; LAD-1; Linear IgA bullous disease antigen of 97 kDa; Linear IgA disease antigen 1; type XVII collagen alpha-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG