CST, 67363S, HA-Tag (6E2) Mouse Monoclonal Antibody (Pacific Blue™ Conjugate)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, HA-Tag (6E2) Mouse Monoclonal Antibody (Pacific Blue^™ Conjugate) (CST #67363) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HA-Tag (6E2) Mouse Monoclonal Antibody (Pacific Blue™ Conjugate) detects recombinant proteins containing the HA epitope tag. This antibody conjugate recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1