CST, 67452S, Cathepsin A (F8X9E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cathepsin A. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cathepsin A (F8X9E) Rabbit Monoclonal Antibody (CST #67452) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Cathepsin A (F8X9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total cathepsin A protein. This antibody detects the full-length (inactive) cathepsin A precursor in addition to the processed (active) light chain. This antibody detects a 75 kDa protein of unknown identity in some cell lines by western blotting. Non-specific nuclear staining was observed in testis by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human cathepsin A protein. Background Cathepsin A, also known as lysosomal protective protein for beta-galactosidase (PPGB), functions as both a serine protease and a critical enzyme for stabilizing β-galactosidase and neuraminidase (1,2). Similar to other cathepsin family members, cathepsin A is synthesized as an inactive 54 kDa precursor, then cleaved into catalytically active large (32 kDa) and small (20 kDa) subunits that form a disulfide-linked heterodimer after excision of a linker peptide (1-3). Cathepsin A degradation targets include endothelin (4), LAMP2 (5), and angiotensin I (6). gene mutations result in protein misfolding and lysosomal galactosialidosis, as neuraminidase and β-galactosidase activity depend on direct association with cathepsin A (7,8). Cathepsin A overexpression in heart causes excess degradation of superoxide dismutase (SOD), increasing oxidative stress, inflammation, and remodeling of the extracellular matrix (ECM) (9). High expression of cathepsin A has been reported and associated with metastasis in melanocytic tumors, colorectal cancer, hepatocellular carcinoma, and lung adenocarcinoma (10-13). Alternate Names beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; carboxypeptidase Y-like kininase; carboxypeptidase-L; Cathepsin A; CTSA; deamidase; GLB2; GSL; lysosomal carboxypeptidase A; Lysosomal protective protein; Lysosomal protective protein 20 kDa chain; Lysosomal protective protein 32 kDa chain; NGBE; PPCA; PPGB; Protective protein cathepsin A; Protective protein for beta-galactosidase; urinary kininase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 54, 20 Source/Isotype: Rabbit IgG