CST, 67459T, Lysozyme C (E9W6E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LYZ1. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Lysozyme C (E9W6E) Rabbit Monoclonal Antibody (CST #67459) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 54081 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Lysozyme C (E9W6E) Rabbit Monoclonal Antibody recognizes endogenous levels of total Lysozyme C protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human Lysozyme C protein. Background Lysozymes are secreted proteins that have bacteriolytic function which are critical for mammalian innate immune function. All lysozymes function to defend host animals from microbial infection by the hydrolysis of bacterial cell wall peptidoglycan (1). Conventional-type lysozymes (Lysozyme C) are one of three types of lysozymes; each family member is categorized based on amino acid sequence and biochemical properties. Lysozyme C is expressed in mammalian secretions like tears, urine, and milk but is also expressed by phagocytes such as macrophages, neutrophils, and dendritic cells. Lysozyme C is encoded in humans by a single gene. The mouse orthologs of Lysozyme C are encoded by two genes, and , which encode Lysozyme C-1 and Lysozyme C-2 (Lysozyme C-1/2). Interestingly, is upregulated in microglia of Alzheimer's disease mouse model brains that have been stimulated by specific forms of activity (2). and are uniquely expressed in microglia, and increased correlates with microglia-mediated β-amyloid (Aβ) clearance, suggesting that Lysozyme C-1/2 may directly contribute to microglial-clearance of Aβ or act as a marker for certain microglial activity states in the brain (3). Alternate Names 1,4-beta-N-acetylmuramidase C; c-type lysozyme; LYSC; lysozyme; lysozyme (renal amyloidosis); Lysozyme C; lysozyme F1; LYZ; LYZF1; LZM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG