CST, 67499S, CD103/ITGAE (F2C7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD103 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CD103/ITGAE (F2C7B) Rabbit Monoclonal Antibody (CST #67499) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity CD103/ITGAE (F2C7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD103/ITGAE protein. Nonspecific staining was observed in mouse kidney tubules, testis Leydig cells, and forestomach epithelium by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu152 of mouse CD103/ITGAE protein. Background Cluster of differentiation molecule 103 (CD103)/Integrin, alpha E (ITGAE) is a transmembrane protein forming heterodimers that are composed of α and β subunits. CD103/ITGAE is expressed by subsets of CD4 and CD8 T cells, dendritic cells, and mast cells in mucosal tissues (1,2). The heterodimer composed of CD103/ITGAE and integrin beta 7 interacts with E-cadherin, a cellular adhesion molecule found on epithelial cells. The CD103/ITGAE and integrin beta 7 complex plays a role in thymocyte proliferation, intestinal T cell homing, and the antitumor immune response (3-5). Studies have shown that the presence of CD103/ITGAE(+) immune cells can be used as markers for a favorable prognosis in different epithelial cancers (6,7). Alternate Names A530055J10; alph; alpha-E1; alpha-M290; aM290; CD103; integrin alpha E, epithelial-associated; Integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; ITAE; Itgae; MGC183601; OTTMUSP00000006421; OTTMUSP00000006422 Specification REACTIVITY: M SENSITIVITY: Endogenous (IHC-P, IF-F), Transfected (W) MW (kDa): 130 Source/Isotype: Rabbit IgG