CST, 67517SF, SERPINB3 (F3Z8E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SERPINB3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, SERPINB3 (F3Z8E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #67517) is ready to ship. Product Usage Information This product is the carrier free version of product #26614. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 26614 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SERPINB3 (F3Z8E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SERPINB3 protein. This antibody detects a 25 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu323 of human SERPINB3 protein. Background Serpin family B member 3 (SERPINB3), also known as squamous cell carcinoma antigen 1 (SCCA1), belongs to the serpin family and inhibits papain-like cysteine proteases (1). SERPINB3 is unique to the other serpins, as it lacks inhibitory activity against serine proteases but has inhibitory activity against papain-like cysteine proteases cathepsins K, L, and S (1,2). It may function as an inhibitor to modulate the host immune response against tumor cells and can act as a UV-induced apoptosis inhibitor by suppressing the activity of c-Jun NH2-terminal kinase 1 (JNK1) (1,3). SERPINB3 is expressed in normal squamous epithelium and in several organs, including tongue, esophageal, uterine, cervical, and vaginal epithelium. It is also expressed in Hassall's corpuscles of the thymus and some areas of the skin (4). SERPINB3 has been found to be overexpressed and implicated in several types of cancer, including hepatocellular carcinoma (HCC), lung, ovarian, and breast cancer (5-8). It is also overexpressed and implicated in hepatoblastoma, autoimmune disorders, lung fibrosis, cholesteatoma, and skin disease (9-13). Alternate Names HsT1196; Protein T4-A; SCC; SCCA; SCCA-1; SCCA-PD; SCCA1; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; Serpin B3; serpin family B member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; SERPINB3; SPB3; Squamous cell carcinoma antigen 1; SSCA1; T4-A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG