CST, 67774T, Ch-TOG (D2Z8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CKAP5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Ch-TOG (D2Z8J) Rabbit Monoclonal Antibody (CST #67774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ch-TOG (D2Z8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total ch-TOG protein. Based on the amino acid sequence of the peptide antigen, this antibody is expected to recognize all isoforms of ch-TOG. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala31 of human ch-TOG protein. Background Ch-TOG (colonic hepatic tumor overexpressed gene)/CKAP5 (cytoskeleton-associated protein 5) is a microtubule stabilizing protein involved in the organization of mitotic spindle poles through interaction with the transforming acid coiled-coil protein, TACC3 (1). Ch-TOG and TACC3 also interact with the membrane trafficking protein clathrin, and this interaction is thought to be required for clathrin's mitotic function in crosslinking microtubules in the mitotic spindle (2). Researchers have found that expression levels of both TACC3 and ch-TOG are correlated with human diseases such as glioblastoma and hepatic carcinoma (3). A genome-wide siRNA screen identified ch-TOG and other G2/M phase regulators as potential contributors to head and neck squamous cell carcinoma (4). Alternate Names Ch-TOG; CHTOG; CKAP5; Colonic and hepatic tumor over-expressed gene protein; Colonic and hepatic tumor overexpressed gene protein; cytoskeleton associated protein 5; Cytoskeleton-associated protein 5; FLJ35359; KIAA0097; MSPS; TOG; TOGp; XMAP215 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 225 Source/Isotype: Rabbit IgG