CST, 67798S, GBP5 (D3A5O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GBP5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GBP5 (D3A5O) Rabbit Monoclonal Antibody (CST #67798) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GBP5 (D3A5O) Rabbit Monoclonal Antibody recognizes endogenous levels of total GBP5 protein. Depending on Western blotting conditions, GBP5 is sometimes observed as a doublet. This is likely due to cleavage of a predicted three amino acid propeptide sequence. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala561 of human GBP5 protein. Background GBP5 (guanylate binding protein 5) is one of seven interferon-inducible GTPases in humans that have recently been shown to be involved in host defense against intracellular pathogens (1,2). Specifically, in response to intracellular bacteria or cell wall components, GBP5 acts as a tetramer to facilitate assembly of the NLRP3 inflammasome, leading to caspase-1 activation (2,3). In addition, GBP5 enables activation of the AIM2 inflammasome by promoting lysis of intracellular bacteria and release of pathogenic double-stranded DNA (4). Alternate Names GBP-5; GBP-TA antigen; GBP5; GTP-binding protein 5; Guanine nucleotide-binding protein 5; guanylate binding protein 5; Guanylate-binding protein 5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 67 Source/Isotype: Rabbit IgG