CST, 67825T, DeltaN p63 (E6Q3O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying deltaNp63. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, DeltaN p63 (E6Q3O) Rabbit Monoclonal Antibody (CST #67825) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77441 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity DeltaN p63 (E6Q3O) Rabbit Monoclonal Antibody recognizes endogenous levels of total DeltaN p63-alpha protein. This antibody is expected to recognize all DeltaN p63 isoforms. This antibody does not detect full length (TA) p63. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala8 of human DeltaN p63 protein. Background The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a truncated ΔNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4). Alternate Names AIS; amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; deltaN-alpha; deltaNp63 alpha; EEC3; Keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; p51delNalpha; p53CP; P63; p63 iso2; P73H; P73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; Tumor protein 63; tumor protein p53-competing protein; tumor protein p63; Tumor protein p73-like Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60, 75, 95 Source/Isotype: Rabbit IgG