CST, 67840S, FUS/TLS (E3O8I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP P2. Validated for WB,IP,IF,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, FUS/TLS (E3O8I) Rabbit Monoclonal Antibody (CST #67840) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:800 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier-free (BSA and azide free) version of this product see product # 48647 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FUS/TLS (E3O8I) Rabbit Monoclonal Antibody recognizes endogenous levels of total FUS/TLS protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln146 of human FUS/TLS protein. Background FUS/TLS (fused in sarcoma/translocated in liposarcoma) was initially identified by investigators as a component of fusion proteins found in a variety of cancers, such as myxoid liposarcoma, acute myeloid leukemia, and Ewing's tumor (1). FUS/TLS fusion with the DNA-binding domain of transcription activators, such as CHOP and ERG, leads to aberrant transcription of target genes that is thought by researchers to lead to tumor development (1-5). FUS/TLS is involved in a wide range of RNA processing events, such as pre-mRNA splicing, mRNA transcription, and miRNA processing (1,6). In addition to its role in RNA metabolism, FUS/TLS maintains genomic stability and co-regulates gene expression by interacting with various transcription factors such as nuclear receptors, YB-1, p65 subunit of NF-κB, TFIID, and RUNX2 (1,6,7). More recently, researchers have found several mutations of FUS/TLS in ALS (amyotrophic lateral sclerosis) and FTLD (frontotemporal lobar degeneration) patients that causes cytoplasmic mislocalization of FUS/TLS (6,8-12). Alternate Names 75 kDa DNA-pairing protein; ALS6; ETM4; FUS; FUS RNA binding protein; fus-like protein; FUS1; fused in sarcoma; fusion gene in myxoid liposarcoma; heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; HNRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA-binding protein FUS; TLS; Translocated in liposarcoma protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG